REVIEW FOR MICROBIOLOGY PRACTICUM

• STUDENTS ARE REPONSIBLE FOR ALL LAB MATERIALS THEY HAVE DEALT SO FAR.
• THE FOLLOWINGS ARE SOME OF TOPICS THEY WILL BE ASKED ON PRACTICUM BUT NOT LIMITED.

COLOR PLATES FROM LAB MANUAL

• Staining plate I
• Microbial metabolism (plate III): omit III.5, III.7, III.10, III.11, III.12
• Plate cultures plate V.5
• Pathology plate VII.3
• Medical microbiology plate VII. 1 and VII.2
• Plate culture for enterics plate XI. 1 and XI.2
• Phototrophic microorganisms plate XIII

MICROSCOPIC SLIDES

• Basic bacteria morphology (rods, cocci, spiral)
• Protozoa – 3 different phyla (Fig. 37.1, Fig. 2.3)
• Green algae – Ex. 36, Table 36.1
• Fungi – Ex. 35, Table 35.1, use pictures on textbook as reference
• different phyla, different spores
• Staining – all staining techniques including
• structural staining,
• function of each structure,
• staining procedures,
• staining reagents,
• staining reactions,
• fixing

MICROSCOPE

• Parts and functions of microscope
• Different types of microscope
• Proper care of microscope

CULTIVATION OF BACTERIA

• Different types of media (plate, broth, agar deep, slant)
• Special media: enrichment, differential, selective
• Specific bacteria that are associated with each type esp. Differential and Selective

MICROBIAL GROWTH

• Methods to cultivate anaerobes
• Interpretation of thyglycollate media
• Temperature
• Difference bet. Fungi and bacteria in osmotic pressure and pH
• Staph. aureus as halophiles

MICROBIAL METABOLISM

• Difference bet. Catabolism and anabolism
• All pH indicators used in each test
• Pos/Neg. Coloric reactions and interpretation such as production of lactic acid or acetoin production etc.
• All reagents required to be added to read the results such as mrvp test, etc.
• Should be able to distinguish different biochemical tests by looking at different media except mrvp test. Examples: glucose media: either with mineral oil or w/o oil
• slant: phyneylalanine deamination slant
• deep: peptone iron agar deep
• fermentation: small inverted tube to trap gas
• etc.
• Enzymes involved in reactions
• Phyneylalanine deamination, orinithin decarboxylation, starch hydrolysis, gelatin hydrolysis, urea, indole, etc.
• End products such as ammonia

INTERACTIONS OF MICROBE AND HOST

• Disinfectants, antiseptics, antibiotics
• Zone of inhibition
• MIC
• Susceptibility / resistance

BACTERIAL GENETICS

• Examples of phenotypes that we tested in the lab associated with specific bacteria (color pigmentation)
• Genotypes / Phenotypes
• Gene regulation:
• Growth of HB 101 vs. HB101 pGLO on LB, LB-Amo & LA-Amp-Ara plates

UV EXPERIEMENT

• Photoreactivation- what it is and how it works. Enzyme
• Dark repair mechanism
• Should be able to interpret results